Pseudomonas fluorescens

How to Guide to Characterizing your Unknown For your unknown, you should find all of the following information before your start writing your report: - Gram reaction - Shape and arrangement of organism - Catalase test (for Gram + only) - All media needed for ID (use the Gram + and – ID labs) and descriptions of any color changes Checklist for your Unknown Report: This is your original work – Show off what you have learned this semester! :-) Please don’t copy and paste passages from the internet/books/articles. Plagiarism (or more then 40% similarity) will result in at least 10 point deduction. Reports that have a higher than 50% similarity will receive 25 points deduction. This report should be in full sentences (Methods excluded) Title (5 Points) - Describes in a few words what was done - Is short and precise (no longer than one sentence) Introduction/Background (20 points) - Describe the purpose of Microbiological Testing o Why is testing important in the medical field? o What is the goal of microbiological testing? - Background on Gram negatives and Gram positives o Differences between Gram + and – (color doesn’t count…) o Give some examples of each o Describe at least two diseases for each, signs, symptoms Methods (15) - Describe the Gram staining procedure - Describe how catalase test would have been done (if this test was used) - Name all media that should have been inoculated to ID your unknown and why Results (20 points) - Describe results for Gram reaction, cell shape and arrangement (look up information) - What color changes were observed for each test? Any zones of inhibition (for antibiotics only)? o Use pix of media found in the Gram + and – powerpoints for this - Choose a good microscopic picture (e.g. Gram stained organism) found on the web but you must reference the source. Discussion (20 points) - Explain how your organism’s tests differed from the other gram – or + - Write at least two paragraph about the significance of your organism o e.g. Diseases it causes, Interesting facts etc. (The information can be collected from sources such as your text book, lab manual, or internet.) References (5 points). - Contains a list of sources you used for your report. You should always give credit to a source. Overall organization (5 points) - Report is easy to follow and contains all the subcategories in a logical order. - Grammar and spelling counts Submit electronic copy on due date (5 points) - Late reports will receive 0/5 points in this category.